hndf cells  (PromoCell)

Journal: Stem Cell Research & Therapy

Article Title: Adipose-derived endothelial and mesenchymal stem cells enhance vascular network formation on three-dimensional constructs in vitro

doi: 10.1186/s13287-015-0251-6

Figure Lengend Snippet: In vitro vessel-like network formation. Confocal images (scale bars = 1000 μm) of whole-mount PLLA/PLGA scaffolds embedded with a HAMECs and MSCs, b HUVECs and MSCs, c HAMECs and HNDFs or d HUVECs and HNDFs and stained for CD31 ( red ) and nuclei ( blue ), on days 4, 7, 10 and 14 post-seeding. HAMEC Human adipose microvascular endothelial cell, HNDF Human neonatal dermal fibroblast, HUVEC Human umbilical vein endothelial cell, MSC Mesenchymal stem cell

Article Snippet: Human foreskin fibroblast cells (HNDFs; Lonza) were cultured in DMEM (Gibco Life Technologies) supplemented with 10 % FBS (Hyclone; Thermo Fisher Scientific), 1 % nonessential amino acids, and 0.2 % β-mercaptoethanol (Sigma-Aldrich).

Techniques: In Vitro, Staining

Journal: Stem Cell Research & Therapy

Article Title: Adipose-derived endothelial and mesenchymal stem cells enhance vascular network formation on three-dimensional constructs in vitro

doi: 10.1186/s13287-015-0251-6

Figure Lengend Snippet: Vessel-like network development in vitro. a Schematic illustration of four stages of developing vessels: single cells, clusters, initial vessels and advanced vessels. The stages for development were manually scored using a percentage scale (0–100 %), where a higher percentage was given to more developed vessel-like networks. b Comparison of vessel development in scaffolds bearing different cell combinations, assessed at different time points in culture. Two-way ANOVA, p value = 0.0297, n ≥ 4. * p < 0.05, ** p < 0.01. HAMEC Human adipose microvascular endothelial cell, HNDF Human neonatal dermal fibroblast, HUVEC Human umbilical vein endothelial cell, MSC Mesenchymal stem cell

Article Snippet: Human foreskin fibroblast cells (HNDFs; Lonza) were cultured in DMEM (Gibco Life Technologies) supplemented with 10 % FBS (Hyclone; Thermo Fisher Scientific), 1 % nonessential amino acids, and 0.2 % β-mercaptoethanol (Sigma-Aldrich).

Techniques: In Vitro, Comparison

Journal: Stem Cell Research & Therapy

Article Title: Adipose-derived endothelial and mesenchymal stem cells enhance vascular network formation on three-dimensional constructs in vitro

doi: 10.1186/s13287-015-0251-6

Figure Lengend Snippet: Representative images of AngioTool analysis and average vessel length determination. a Representative images (scale bars = 250 μm) of i single cells and ii developed vessel-network embedded within scaffolds, as captured through a confocal microscope. b The AngioTool program marks the i single cells ( red ) or ii developed vessels ( red ), junctions ( blue ) and vessel borders ( yellow ). c Average vessel length for different cell combinations was then determined. Two-way ANOVA, p value = 0.016, n ≥ 3. * p < 0.05, *** p < 0.001. HAMEC Human adipose microvascular endothelial cell, HNDF Human neonatal dermal fibroblast, HUVEC Human umbilical vein endothelial cell, MSC Mesenchymal stem cell

Article Snippet: Human foreskin fibroblast cells (HNDFs; Lonza) were cultured in DMEM (Gibco Life Technologies) supplemented with 10 % FBS (Hyclone; Thermo Fisher Scientific), 1 % nonessential amino acids, and 0.2 % β-mercaptoethanol (Sigma-Aldrich).

Techniques: Microscopy

Journal: Stem Cell Research & Therapy

Article Title: Adipose-derived endothelial and mesenchymal stem cells enhance vascular network formation on three-dimensional constructs in vitro

doi: 10.1186/s13287-015-0251-6

Figure Lengend Snippet: Distribution of complexity factor ranges for the different cell pairs. The complexity factor of networks within cell-embedded scaffolds was determined on days 4, 7, 10 and 14 post-seeding. The distribution of complexity factors per cell pair and per day of analysis is presented. a Day 4 ( p < 0.001. b Day 7 ( p = 0.003). c Day 10 ( p = 0.055). d Day 14 ( p < 0.001). Two-way ANOVA, n ≥ 3. * p < 0.05, ** p < 0.01, *** p < 0.001. HAMEC Human adipose microvascular endothelial cell, HNDF Human neonatal dermal fibroblast, HUVEC Human umbilical vein endothelial cell, MSC Mesenchymal stem cell

Article Snippet: Human foreskin fibroblast cells (HNDFs; Lonza) were cultured in DMEM (Gibco Life Technologies) supplemented with 10 % FBS (Hyclone; Thermo Fisher Scientific), 1 % nonessential amino acids, and 0.2 % β-mercaptoethanol (Sigma-Aldrich).

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Adipose-derived endothelial and mesenchymal stem cells enhance vascular network formation on three-dimensional constructs in vitro

doi: 10.1186/s13287-015-0251-6

Figure Lengend Snippet: Vessel alignment as an indication of vessel maturity. a Representative image of half of a scaffold showing different regions with orientated vessels (scale bar = 500 μm). b Magnified image of vessels orientated in a specific direction (scale bar = 100 μm) and c a histogram showing the distribution of the elements orientation in image b (represented by different angles). d A magnified image of non-oriented vessels (scale bar = 100 μm) and e a histogram showing the distribution of the elements orientation in image d (represented by different angles). f Statistical analysis of the number of elements in a given direction versus the total number of elements (%) in each field of view. Two-way ANOVA, Bonferroni’s multiple comparisons test, n ≥ 4. **** p < 0.0001. HAMEC Human adipose microvascular endothelial cell, HNDF Human neonatal dermal fibroblast, HUVEC Human umbilical vein endothelial cell, MSC Mesenchymal stem cell

Article Snippet: Human foreskin fibroblast cells (HNDFs; Lonza) were cultured in DMEM (Gibco Life Technologies) supplemented with 10 % FBS (Hyclone; Thermo Fisher Scientific), 1 % nonessential amino acids, and 0.2 % β-mercaptoethanol (Sigma-Aldrich).

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Adipose-derived endothelial and mesenchymal stem cells enhance vascular network formation on three-dimensional constructs in vitro

doi: 10.1186/s13287-015-0251-6

Figure Lengend Snippet: Evaluation of vessel maturity using cell markers. a Paraffin-embedded sections (5 μm) of cell-embedded PLLA/PLGA scaffolds were fluorescently labeled for i α-SMA ( red ) and ii CD31 ( green ) (scale bars = 20 μm). iii A merged image is presented for α-SMA and CD31 staining (scale bar = 20 μm). b Statistical analysis of α-SMA coverage out of the overall lumen perimeter, determined by CD31 staining, was performed for vessels under 35 μm in diameter. Two-way ANOVA, Tukey’s multiple comparisons test, n ≥ 4. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. α-SMA Alpha-smooth muscle actin, HAMEC Human adipose microvascular endothelial cell, HNDF Human neonatal dermal fibroblast, HUVEC Human umbilical vein endothelial cell, MSC Mesenchymal stem cell

Article Snippet: Human foreskin fibroblast cells (HNDFs; Lonza) were cultured in DMEM (Gibco Life Technologies) supplemented with 10 % FBS (Hyclone; Thermo Fisher Scientific), 1 % nonessential amino acids, and 0.2 % β-mercaptoethanol (Sigma-Aldrich).

Techniques: Labeling, Staining